华北农学报 ›› 2019, Vol. 34 ›› Issue (1): 19-25. doi: 10.7668/hbnxb.201751117

所属专题: 番茄 抗旱节水 生物技术

• 作物遗传育种·种质资源·生物技术 • 上一篇    下一篇

番茄SlETR6基因的克隆及非生物胁迫下的表达分析

苏丽艳   

  1. 西安文理学院 生物与环境工程学院, 秦岭野生观赏植物研究中心, 陕西 西安 710065
  • 收稿日期:2018-11-02 出版日期:2019-02-28
  • 作者简介:苏丽艳(1983-),女,河北迁安人,讲师,博士,主要从事植物发育与分子调控机理研究。
  • 基金资助:
    国家自然科学基金项目(31701935);陕西省自然科学基础研究计划(2017JQ3009);陕西省教育厅自然科学专项(17JK1126);西安市科技计划项目(2016CXWL03)

Cloning and Expression Analysis of Ethylene Receptor Gene SlETR6 in Solanum lycopersicum under Abiotic Stress

SU Liyan   

  1. School of Biological and Environmental Engineering, Xi'an University, Research Center for Qinling Wild Ornamental Plant, Xi'an 710065, China
  • Received:2018-11-02 Published:2019-02-28

摘要: 为解析番茄乙烯受体基因SlETR6在非生物胁迫过程中的功能。以番茄为材料,克隆了番茄SlETR6基因,利用MEGA 5.0软件对SlETR6基因编码的蛋白进行了聚类分析,并利用实时荧光定量PCR分析了SlETR6基因在番茄根、叶、花、果实不同发育时期的组织表达情况及对其在高盐、高温(40℃)、低温(4℃)、干旱胁迫条件下的表达模式。结果表明,SlETR6基因开放阅读框(Open reading frame,ORF)为2 265 bp,编码754个氨基酸,蛋白质分子质量为85.05 ku,等电点为7.28,与马铃薯StETR-like蛋白的同源性最高;启动子分析显示,SlETR6基因含有热胁迫、干旱胁迫、低氧胁迫、光响应、乙烯、水杨酸及赤霉素应答等相关的顺式作用元件。实时荧光定量PCR分析表明,SlETR6在番茄不同组织中均有表达,且在花和转色期果实中有显著高表达。高盐胁迫6 h后,SlETR6基因呈现上调表达,12 h后达到峰值,而后回复正常表达水平;高温胁迫后,SlETR6基因表达量有明显的上升趋势;干旱胁迫早期SlETR6基因应答强烈,在胁迫处理1 h后表达量达到峰值。因此,推测SlETR6基因可能在番茄适应高盐、高温、干旱等逆境胁迫过程中发挥重要作用,可能参与番茄生长发育过程中的逆境调控。为番茄抗逆研究提供了新的候选基因资源。

关键词: 番茄, SlETR6, 基因克隆, 非生物胁迫, 表达分析

Abstract: In order to identify the function of SlETR6 gene in tomato under different abiotic stresses, the SlETR6 gene was cloned by RT-PCR from Solanum lycopersicum, and the phylogenetic tree was clustered based on its amino acids by MEGA 5.0 soft. The temporal and spatial (included root, leaf, flower, and fruit in different stage) expression patterns of SlETR6 were analyzed by Real-time quantitative PCR (qPCR), and the relative expression level of SlETR6 gene was analyzed by qPCR under high salt stress, high temperature, low temperature and drought stress during tomato seedling development. The results showed that SlETR6 contained 2 265 bp ORFs, encoded 754 amino acid residues. The protein SlETR6 with a molecular mass of 80.05 ku and isoelectric point of 7.28, and had a closer relationship with Solanum tuberosum ETR2-like. Promoter analysis revealed that SlETR6 contained several stress-responsive elements involved in heat, drought, anaerobic, light, ethylene, salicylic acid, and gibberellin signaling. qPCR analysis showed that the SlETR6 was constitutively expressed in all examined tissues, including seed, root, flower, leaf and fruit,and it showed a higher level in flower and breaker fruit. SlETR6 was up-regulated under high salt stress 6 h compare to control, and the expression level was the highest at 12 h under stress, then return to the normal. The transcription level of SlETR6 was up-regulated significantly under high-temperature stress. Drought stress could significantly up-regulated the expression of SlETR6 in the early stage, and the expression level was the highest at 1 h under stress. These results suggested that SlETR6 might function as an stress-responsive gene under high salt stress, high temperature and drought stress during tomato development. This study provided a potential novel candidate for abiotic research in tomato.

Key words: Tomato, SlETR6, Gene clone, Abiotic stress, Expression analysis

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引用本文

苏丽艳. 番茄SlETR6基因的克隆及非生物胁迫下的表达分析[J]. 华北农学报, 2019, 34(1): 19-25. doi: 10.7668/hbnxb.201751117.

SU Liyan. Cloning and Expression Analysis of Ethylene Receptor Gene SlETR6 in Solanum lycopersicum under Abiotic Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 19-25. doi: 10.7668/hbnxb.201751117.

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