华北农学报 ›› 2018, Vol. 33 ›› Issue (5): 82-88. doi: 10.7668/hbnxb.2018.05.011

所属专题: 棉花 生物技术

• 论文 • 上一篇    下一篇

棉花GhTGA1GhTGA9.2基因克隆及表达特征分析

赵曾强2, 李潇玲1, 张析1, 张薇1, 练文明3   

  1. 1. 石河子大学 农学院, 新疆 石河子 832000;
    2. 新疆农垦科学院 生物技术研究所, 作物种质创新与基因资源 利用兵团重点实验室, 新疆 石河子 832000;
    3. 新疆生产建设兵团第一师农业科学研究所, 新疆 阿拉尔 843301
  • 收稿日期:2018-07-01 出版日期:2018-10-28
  • 通讯作者: 练文明(1974-),男,新疆阿克苏人,副研究员,主要从事棉花育种与栽培研究。
  • 作者简介:赵曾强(1985-),男,甘肃静宁人,助理研究员,硕士,主要从事棉花分子育种研究。
  • 基金资助:
    国家自然科学基金项目(31260358)

Cloning and Expression Analysis of GhTGA1 and GhTGA9.2 in Cotton

ZHAO Zengqiang2, LI Xiaoling1, ZHANG Xi1, ZHANG Wei1, LIAN Wenming3   

  1. 1. Agricultural College, Shihezi University, Shihezi 832000, China;
    2. Institute of Biotechnology, Xinjiang Academy of Agricultural Reclamation Sciences, Xinjiang Production & Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Shihezi 832000, China;
    3. Agricultural Science Institute,1st Agricultural Production Division, Xinjiang Production and Construction Corps, Aral 843301, China
  • Received:2018-07-01 Published:2018-10-28

摘要: 为了揭示棉花GhTGA1GhTGA9.2基因的生物学功能,利用RT-PCR技术,从抗枯萎病棉花品种中棉所12号中克隆了TGA转录因子基因GhTGA1GhTGA9.2。生物信息学分析表明,2个基因开放阅读框(ORF)长分别为1 281,1461 bp,分别编码405,486个氨基酸。序列分析表明,2个基因均含有bZIP和DOG1结构域,属于bZIP亚家族TGA转录因子基因。利用实时荧光定量(qRT-PCR)技术进行表达特征分析表明,GhTGA1基因能被枯萎病菌、乙烯(ET)、茉莉酸(JA)诱导上调表达,水杨酸(SA)诱导后,在各处理时间点,该基因表达量均低于Mock,以上结果表明,GhTGA1基因可能通过参与乙烯(ET)和茉莉酸(JA)信号通路调控棉花对枯萎病的抗性;枯萎病处理后,GhTGA9.2基因在各时间点表达量均低于Mock,呈下调表达,但能被激素(ET、JA、SA)诱导上调表达。结果表明,GhTGA9.2基因可能通过激素(ET、JA、SA)途径参与胁迫应答,通过对GhTGA1GhTGA9.2基因表达特征分析为后续研究提供基础。

关键词: 棉花, TGA转录因子, GhTGA1GhTGA9.2克隆, 表达分析

Abstract: GhTGA1 and GhTGA9.2 of transcription factor genes were isolated by RT-PCR technology from Zhongmiansuo 12 cultivar of resistance to Fusarium oxysporum. Sequence analysis showed that the ORF of GhTGA1 and GhTGA9.2 were 1 281 bp and 1 461 bp,respectively.They encoded 405 and 486 amino acids,respectively. GhTGA1 and GhTGA9.2 all contained two conserved bZIP and DOG1 domains. They belonged to the TGA transcription factor gene of bZIP subfamily. The GhTGA1 and GhTGA9.2 expression feature were detected by Real-time quantitative PCR(qRT-PCR),the results showed that GhTGA1 gene was up-regulation expressed with the duration of the treatment of Fov,ethylene (ET) and jasmonic acid (JA). In each treatment time point,the expression of GhTGA1 gene was lower than that of Mock with the treatment of salicylic acid (SA). We speculated that GhTGA1 gene might be involved in ethylene (ET) and jasmonic acid (JA) signaling pathway and regulated resistance to Fusarium oxysporum in cotton; After the treatment of Fusarium oxysporum,the expression of GhTGA9.2 gene was lower than Mock at different treatment time points,and was down-regulated expression. In other hand,it was up-regulation expressed with the duration of the treatment of hormone (ET,JA,SA).It suggested that GhTGA9.2 might participate in stress response through hormone (ET,JA and SA) pathways. The analysis of gene expression characteristics of GhTGA1 and GhTGA9.2 provides a basis for further study.

Key words: Cotton, TGA transcription factor, Gene clone of GhTGA1 and GhTGA9.2, Expression analysis

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引用本文

赵曾强, 李潇玲, 张析, 张薇, 练文明. 棉花GhTGA1GhTGA9.2基因克隆及表达特征分析[J]. 华北农学报, 2018, 33(5): 82-88. doi: 10.7668/hbnxb.2018.05.011.

ZHAO Zengqiang, LI Xiaoling, ZHANG Xi, ZHANG Wei, LIAN Wenming. Cloning and Expression Analysis of GhTGA1 and GhTGA9.2 in Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 82-88. doi: 10.7668/hbnxb.2018.05.011.

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