摘要： 为有效利用ISSR技术开展华仁杏种质资源与遗传多样性研究,通过L16(4)正交试验设计,对华仁杏ISSRPCR反应体系中的个主要因素进行了最优条件的筛选试验,借助极差分析和方差分析,建立了华仁杏最佳的ISSRPCR反应体系(2μL):10×PCR Buffer without MgC12 2.μL、MgC12 2.0 mmol⁄L、Taq DNA Polymerase 2.0 U、dNTP MiX 100μmol⁄L、ISSR Primer 0.2μmol⁄L、DNA 2.4 ng⁄μL.

关键词: 华仁杏, 杏属, ISSR-PCR反应体系, 正交设计

Abstract: Armeniaca cathayana D.L.Fu et a1.is anew species described in 2010 and has high vs]ues for its big and not bitter kernels.In order to research its germ plasma and genetic diversity based on ISSR technique,the orthogohal design of L16(45)was used to optimize the ISSR-PCR amplification system of A.cathayana in five major factors. Based on range analysis and variance analysis,the best ISSR-PCR reaction system(25 ILL)was established：10×PCR Buffer without MgCl2 2.5 p.L,MgCl2 2.0 mmol/L,Taq DNA Polymerase 2.0 U,dNTP MiX 100 p.mol/L,ISSR Primer 0.2 Ixmol/L,DNA 2.4 ns/L.

Key words: Armeniaca cathayana, Armeniaca Scop, ISSR-PCR amplification system, Orthogonal design

中图分类号: 

• S662.2